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 | Acesso ao texto completo restrito à biblioteca da Embrapa Trigo. Para informações adicionais entre em contato com cnpt.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
17/02/2021 |
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Data da última atualização: |
17/02/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
POLONI, N. M.; CARVALHO, G.; VICENTINI, S. N. C.; DORIGAN, A. F.; MACIEL, J. L. N.; MCDONALD, B. A.; MOREIRA, S. I.; HAWKINS, N.; FRAAIJE, B. A.; KELLY, D. E.; KELLY, S. L.; CERESINI, P. C. |
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Afiliação: |
NADIA MARIA POLONI, Department of Crop Protecion, Agricultural Engineering, and Soil (Departamento de Fitossanidade, Engenharia Rural e Solos), São PAulo State University, UNESP; GISELLE CARVALHO, Department of Crop Protecion, Agricultural Engineering, and Soil (Departamento de Fitossanidade, Engenharia Rural e Solos), São PAulo State University, UNESP; SAMARA NUNES CAMPOS VICENTINI, Department of Crop Protecion, Agricultural Engineering, and Soil (Departamento de Fitossanidade, Engenharia Rural e Solos), São PAulo State University, UNESP; ADRIANO FRANCIS DORIGAN, 1Department of Crop Protection, Agricultural Engineering, and Soil (Departamento de Fitossanidade, Engenharia Rural e Solos), São Paulo State University , UNESP, Ilha Solteira, São Paulo, Brazil; JOAO LEODATO NUNES MACIEL, CNPT; BRUCE A. MCDONALD, Plant Pathology Group, Institute of Integrative Biology, ETH Zürich, Zürich, Switzerland; SILVINO INTRA MOREIRA, 1Department of Crop Protection, Agricultural Engineering, and Soil (Departamento de Fitossanidade, Engenharia Rural e Solos), São Paulo State University , UNESP, Ilha Solteira, São Paulo, Brazil; NICHOLA HAWKINS, Biointeractions and Crop Protection, Rothamsted Research, Harpenden, UK; BART A. FRAAIJE, Biointeractions and Crop Protection, Rothamsted Research, Harpenden, UK; DIANE E. KELLY, 6Centre for Cytochrome P450 Biodiversity, Institute of Life Science, School of Medicine, Swansea University, Swansea, UK; STEVEN L. KELLY, 6Centre for Cytochrome P450 Biodiversity, Institute of Life Science, School of Medicine, Swansea University, Swansea, UK; PAULO C. CERESINI, 1Department of Crop Protection, Agricultural Engineering, and Soil (Departamento de Fitossanidade, Engenharia Rural e Solos), São Paulo State University , UNESP, Ilha Solteira, São Paulo, Brazil. |
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Título: |
Widespread distribution of resistance to triazole fungicides in brazilian populations of the wheat blast pathogen. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plant Pathology, v. 1, p. ppa.13288, Sept. 2020. |
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DOI: |
10.1111/ppa.1328 |
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Idioma: |
Inglês |
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Conteúdo: |
Fungicides have not been effective in controlling the wheat blast disease in Brazil. An earlier analysis of 179 isolates of Pyricularia oryzae Triticum lineage (PoTl) sampled from wheat fields across six populations in central-southern Brazil during 2012 discovered a high level of resistance to strobilurin fungicides. Here we analysed azole resistance in the same strains based on EC50 measurements for tebuconazole and epoxiconazole. All six Brazilian populations of PoTl exhibited high resistance to both azoles, with in vitro EC50 values that were at least 35 to 50 times higher than the recommended field doses. We sequenced the CYP51A and CYP51B genes to determine if they were likely to play a role in the observed azole resistance. Although we found five distinct haplotypes in PoTl carrying four nonsynonymous substitutions in CYP51A, none of these substitutions were correlated with elevated EC50. CYP51B was sequenced for nine PoTl isolates, three each representing low, medium, and high tebuconazole EC50. Both PoTl CYP51A and CYP51B could complement yeast CYP51 function. All PoTl CYP51A-expressing yeast transformants were less sensitive to triazoles than the PoTl CYP51B ones. Transformants expressing PoTl CYP51A haplotype H1 carrying the R158K substitution were not more resistant than those expressing PoTl CYP51A haplotype H5, which is synonymous to haplotype H6, found in triazole-sensitive P. oryzae Oryza isolates from rice blast. Therefore, the reduced triazole sensitivity of wheat blast isolates compared to rice blast isolates appears to be associated with a non-target-site related resistance mechanism acquired after higher exposure to triazoles. MenosFungicides have not been effective in controlling the wheat blast disease in Brazil. An earlier analysis of 179 isolates of Pyricularia oryzae Triticum lineage (PoTl) sampled from wheat fields across six populations in central-southern Brazil during 2012 discovered a high level of resistance to strobilurin fungicides. Here we analysed azole resistance in the same strains based on EC50 measurements for tebuconazole and epoxiconazole. All six Brazilian populations of PoTl exhibited high resistance to both azoles, with in vitro EC50 values that were at least 35 to 50 times higher than the recommended field doses. We sequenced the CYP51A and CYP51B genes to determine if they were likely to play a role in the observed azole resistance. Although we found five distinct haplotypes in PoTl carrying four nonsynonymous substitutions in CYP51A, none of these substitutions were correlated with elevated EC50. CYP51B was sequenced for nine PoTl isolates, three each representing low, medium, and high tebuconazole EC50. Both PoTl CYP51A and CYP51B could complement yeast CYP51 function. All PoTl CYP51A-expressing yeast transformants were less sensitive to triazoles than the PoTl CYP51B ones. Transformants expressing PoTl CYP51A haplotype H1 carrying the R158K substitution were not more resistant than those expressing PoTl CYP51A haplotype H5, which is synonymous to haplotype H6, found in triazole-sensitive P. oryzae Oryza isolates from rice blast. Therefore, the reduced triazole sensitivity o... Mostrar Tudo |
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Palavras-Chave: |
CYP51A; CYP51B; Epoxiconazole; Pyricularia oryzae Oryza lineage; Pyricularia oryzae Triticum lineage. |
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Thesaurus Nal: |
Tebuconazole. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02669naa a2200337 a 4500
001 2130037
005 2021-02-17
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1111/ppa.1328$2DOI
100 1 $aPOLONI, N. M.
245 $aWidespread distribution of resistance to triazole fungicides in brazilian populations of the wheat blast pathogen.$h[electronic resource]
260 $c2020
520 $aFungicides have not been effective in controlling the wheat blast disease in Brazil. An earlier analysis of 179 isolates of Pyricularia oryzae Triticum lineage (PoTl) sampled from wheat fields across six populations in central-southern Brazil during 2012 discovered a high level of resistance to strobilurin fungicides. Here we analysed azole resistance in the same strains based on EC50 measurements for tebuconazole and epoxiconazole. All six Brazilian populations of PoTl exhibited high resistance to both azoles, with in vitro EC50 values that were at least 35 to 50 times higher than the recommended field doses. We sequenced the CYP51A and CYP51B genes to determine if they were likely to play a role in the observed azole resistance. Although we found five distinct haplotypes in PoTl carrying four nonsynonymous substitutions in CYP51A, none of these substitutions were correlated with elevated EC50. CYP51B was sequenced for nine PoTl isolates, three each representing low, medium, and high tebuconazole EC50. Both PoTl CYP51A and CYP51B could complement yeast CYP51 function. All PoTl CYP51A-expressing yeast transformants were less sensitive to triazoles than the PoTl CYP51B ones. Transformants expressing PoTl CYP51A haplotype H1 carrying the R158K substitution were not more resistant than those expressing PoTl CYP51A haplotype H5, which is synonymous to haplotype H6, found in triazole-sensitive P. oryzae Oryza isolates from rice blast. Therefore, the reduced triazole sensitivity of wheat blast isolates compared to rice blast isolates appears to be associated with a non-target-site related resistance mechanism acquired after higher exposure to triazoles.
650 $aTebuconazole
653 $aCYP51A
653 $aCYP51B
653 $aEpoxiconazole
653 $aPyricularia oryzae Oryza lineage
653 $aPyricularia oryzae Triticum lineage
700 1 $aCARVALHO, G.
700 1 $aVICENTINI, S. N. C.
700 1 $aDORIGAN, A. F.
700 1 $aMACIEL, J. L. N.
700 1 $aMCDONALD, B. A.
700 1 $aMOREIRA, S. I.
700 1 $aHAWKINS, N.
700 1 $aFRAAIJE, B. A.
700 1 $aKELLY, D. E.
700 1 $aKELLY, S. L.
700 1 $aCERESINI, P. C.
773 $tPlant Pathology$gv. 1, p. ppa.13288, Sept. 2020.
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Embrapa Trigo (CNPT) |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
23/10/2020 |
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Data da última atualização: |
23/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
MOURA, P. F.; AUER, C. G.; RECH, K. S.; OLIVEIRA, C. F. de; OLIVEIRA, C. da S. P. de; HIROTA, B. C. K.; DIAS, J. de F. G.; MIGUEL, O. G.; MIGUEL, M. D. |
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Afiliação: |
Paula Francislaine Moura, UFPR; CELSO GARCIA AUER, CNPF; Katlin Suellen Rech, UFPR; Camila Freitas de Oliveira, UFPR; Cristiane da Silva Paula de Oliveira, UFPR; Beatriz Cristina Konopatzki Hirota, UFPR; Josiane de Fátima Gaspari Dias, UFPR; Obdulio Gomes Miguel, UFPR; Marilis Dallarmi Miguel, UFPR. |
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Título: |
Biotechnology of biomass production in vitro of fungi isolated from Pinus. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Research, Society and Development, v. 9, n.10, e7809109080, 2020. 15 p. |
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DOI: |
http://dx.doi.org/10.33448/rsd-v9i10.9080 |
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Idioma: |
Inglês |
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Conteúdo: |
Fungos são organismos capazes de sintetizar metabólitos de interesse industrial e a padronização da produção de biomassa para a extração desses compostos tem aplicações biotecnológicas.O objetivo deste trabalho foi otimizar o processo de cultivo in vitropara fungosisolados de Pinussp., padronizandoas melhores condições para a produção de biomassa, contribuindo para sua produção em larga escala.Portanto, as condições de cultivo in vitrodos fungos Botrytis cinerea, Rhizoctonia sp. e Suillus sp., foram avaliados com base na produção máxima de biomassa seca (PBS), variando temperatura, meio e tempo de cultivo. Os fungos foram cultivados em frascos de vidro com meios de cultura líquidos, em câmara BOD, sem agitação mecânica. Caldos de batata-dextrose (BD), Czapek (CZ) e extrato de malte (EM) foram avaliadosem temperaturas variando de 8 a 32 ºC e tempos de incubação de 7 a 35 dias. O caldo BDapresentou melhores resultados para os fungos B. cinerea eRhizoctonia sp.,quando comparado com oscaldos CZ e EM, na PBS, enquantoque Suillus sp. apresentou melhor desenvolvimento em caldo EM. A melhor temperatura decrescimento com base no PBS foi de 12 ºC e 16 ºC, com 28 e 35 dias de cultivo. |
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Palavras-Chave: |
Biotechnological optimization; Otimização biotecnológica. |
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Thesagro: |
Biomassa; Botrytis Cinerea; Fungo. |
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Thesaurus NAL: |
Biomass; Fungi; Pinus; Rhizoctonia; Suillus. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216979/1/Celso-Auer-9080-Article-127409-1-10-20201018-3.pdf
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Marc: |
LEADER 02228naa a2200349 a 4500
001 2125821
005 2020-10-23
008 2020 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.33448/rsd-v9i10.9080$2DOI
100 1 $aMOURA, P. F.
245 $aBiotechnology of biomass production in vitro of fungi isolated from Pinus.$h[electronic resource]
260 $c2020
520 $aFungos são organismos capazes de sintetizar metabólitos de interesse industrial e a padronização da produção de biomassa para a extração desses compostos tem aplicações biotecnológicas.O objetivo deste trabalho foi otimizar o processo de cultivo in vitropara fungosisolados de Pinussp., padronizandoas melhores condições para a produção de biomassa, contribuindo para sua produção em larga escala.Portanto, as condições de cultivo in vitrodos fungos Botrytis cinerea, Rhizoctonia sp. e Suillus sp., foram avaliados com base na produção máxima de biomassa seca (PBS), variando temperatura, meio e tempo de cultivo. Os fungos foram cultivados em frascos de vidro com meios de cultura líquidos, em câmara BOD, sem agitação mecânica. Caldos de batata-dextrose (BD), Czapek (CZ) e extrato de malte (EM) foram avaliadosem temperaturas variando de 8 a 32 ºC e tempos de incubação de 7 a 35 dias. O caldo BDapresentou melhores resultados para os fungos B. cinerea eRhizoctonia sp.,quando comparado com oscaldos CZ e EM, na PBS, enquantoque Suillus sp. apresentou melhor desenvolvimento em caldo EM. A melhor temperatura decrescimento com base no PBS foi de 12 ºC e 16 ºC, com 28 e 35 dias de cultivo.
650 $aBiomass
650 $aFungi
650 $aPinus
650 $aRhizoctonia
650 $aSuillus
650 $aBiomassa
650 $aBotrytis Cinerea
650 $aFungo
653 $aBiotechnological optimization
653 $aOtimização biotecnológica
700 1 $aAUER, C. G.
700 1 $aRECH, K. S.
700 1 $aOLIVEIRA, C. F. de
700 1 $aOLIVEIRA, C. da S. P. de
700 1 $aHIROTA, B. C. K.
700 1 $aDIAS, J. de F. G.
700 1 $aMIGUEL, O. G.
700 1 $aMIGUEL, M. D.
773 $tResearch, Society and Development$gv. 9, n.10, e7809109080, 2020. 15 p.
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